文献详情
Harnessing the Periplasm of Bacterial Cells To Develop Biocatalysts for the Biosynthesis of Highly Pure Chemicals
文献类型期刊
作者Yang, Yun[1];Wu, Yichao[2];Hu, Yidan[3];Wang, Hua[4];Guo, Lin[5];Fredrickson, James K.[6];Cao, Bin[7]
机构
通讯作者Guo, L (reprint author), Beihang Univ, Sch Chem, Beijing, Peoples R China.; Guo, L (reprint author), Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Beijing, Peoples R China.; Cao, B (reprint author), Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore, Singapore.; Cao, B (reprint author), Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore, Singapore.
来源信息年:2018  卷:84  期:1  
期刊信息APPLIED AND ENVIRONMENTAL MICROBIOLOGY影响因子和分区  ISSN:0099-2240
关键词biosynthesis; periplasm; flavin mononucleotide; synthetic biology; metabolic engineering; periplasmic space; riboflavin
增刊正刊
摘要Although biocatalytic transformation has shown great promise in chemical synthesis, there remain significant challenges in controlling high selectivity without the formation of undesirable by-products. For instance, few attempts to construct biocatalysts for de novo synthesis of pure flavin mononucleotide (FMN) have been successful, due to riboflavin (RF) accumulating in the cytoplasm and being secreted with FMN. To address this problem, we show here a novel biosynthesis strategy, compartmentalizing the final FMN biosynthesis step in the periplasm of an engineered Escherichia coli strain. This construct is able to overproduce FMN with high specificity (92.4% of total excreted flavins). Such a biosynthesis approach allows isolation of the final biosynthesis step from the cytoplasm to eliminate undesirable byproducts, providing a new route to develop biocatalysts for the synthesis of highpurity chemicals. IMPORTANCE The periplasm of Gram-negative bacterial hosts is engineered to compartmentalize the final biosynthesis step from the cytoplasm. This strategy is promising for the overproduction of high-value products with high specificity. We demonstrate the successful implementation of this strategy in microbial production of highly pure FMN.
收录情况SCIE(WOS:000418039700008)  EI(20180104608124)  PubMed(29079618)  
所属部门化学与环境学院
链接地址https://www.scopus.com/inward/record.uri?eid=2-s2.0-85039854324&doi=10.1128%2fAEM.01693-17&partnerID=40&md5=1efb33bf966f14256342611e8e6922a3
DOI10.1128/AEM.01693-17
百度学术Harnessing the Periplasm of Bacterial Cells To Develop Biocatalysts for the Biosynthesis of Highly Pure Chemicals
语言外文
人气指数372
浏览次数367
基金China Postdoctoral Science Foundation [2016M591043]; Ministry of Education (MOE) Academic Research Fund Tier 1 [M4011622.030]; National Research Foundation; MOE Singapore under its Research Centre of Excellence Programme, Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University (Singapore) [M4330005.C70]
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