文献详情
High-throughput superhydrophobic microwell arrays for investigating multifactorial stem cell niches
文献类型期刊
作者Zhang, Pengfei[1];Zhang, Jianxiong[2];Bian, Shengtai[3];Chen, Zhongyao[4];Hu, Yawei[5];Hu, Ruowen[6];Li, Jiaqi[7];Cheng, Yichun[8];Zhang, Xiaochen[9];Zhou, Yiming[10];Chen, Xiaofang[11];Liu, Peng[12]
机构
通讯作者Liu, P (reprint author), Tsinghua Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Sch Med, Dept Biomed Engn, Beijing 100084, Peoples R China.; Chen, XF (reprint author), Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China.
来源信息年:2016  卷:16  期:16  页码范围:2996-3006  
期刊信息LAB ON A CHIP影响因子和分区  ISSN:1473-0197
增刊正刊
摘要Understanding the complex regulatory network that determines stem cell fates requires a high-throughput platform that can generate a large number of precisely controlled microenvironments representing multiple factors for stem cell culture and analysis. Here, we developed a superhydrophobic microwell array chip on which the culture conditions in each microwell can be spontaneously isolated by a grafted layer of superhydrophobic polymers. Simple steps for medium exchange were developed to facilitate the on-chip culture of both adherent and non-adherent cells for up to six days without compromising cell viability and functionality. The culture conditions in each microwell were facilely manipulated using a robotic spotter. Stem cell niches combining soluble factors, extracellular matrices and microtopographic cues were generated on a single 512-well SMARchip and their combinatorial effects on the fate of mouse Oct4-EGFP iPSCs were systematically probed. We observed significant differences in iPSC pluripotency and proliferation between adherent flat and suspended spherical cultures on our platform, which might provide insights into improvement of stem cell technologies.
收录情况SCIE(WOS:000382144000004)  PubMed(27137909)  
所属部门生物与医学工程学院
DOI10.1039/c6lc00331a
百度学术High-throughput superhydrophobic microwell arrays for investigating multifactorial stem cell niches
语言外文
被引频次66
人气指数27
浏览次数27
基金National High Technology Research and Development Program of China [2015AA020406]; National Natural Science Foundation of China [81301334]
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